Fig. 3

Effect of let-7a mimic and dox on mitochondrial and cellular parameters in MDA-MB-231 and MCF-7 cells. RT-qPCR was used to determine mtDNA level in MDA-MB-231 and MCF-7 cells. mtDNA/nDNA ratio represent mtDNA number in A MDA-MB-231, and B MCF-7 cells. ND4 used for mitochondrial gene was normalised to nuclear β-Actin. Effect of dox and Let-7a on cellular ATP levels in C MDA-MB-231 and D MCF-7 cells. E Flow cytometric analysis of mitotracker green in MCF-7 cells. F and G MCF-7 cells were transfected with mimic/antisense of let-7a for 24 h. Western blot analysis showing expression levels of mitochondrial biogenesis markers TFAM (28KDa) and PGC-1α (100KDa). The data are represented as mean + SD, analyzed by unpaired t-test, n = 3. (p < 0.05). (**p < 0.01), (***p < 0.001), (****p < 0.0001)