Fig. 4
Measurement of oxygen concentration using Oroboros O2K Respirometer. Representative graphs showing measurement of oxygen consumption rate upon addition of different substrates and inhibitor in A MDA-MB-231 cells and B MCF-7 cells. C Measurement of oxygen concentration after combined treatment of 2-DG and let-7a in MDA-MB-231 cells. Bar graphs represents oxygen concentration in ρ0 cells of D MDA-MB-231 and E MCF-7 cells. Measurement of mitochondrial complex I activity in let-7a transfected (mimic/anti) MDA-MB-231 and MCF-7 cells. Data is represented as absorbance versus time. F Showing Complex I activity in MDA-MB-231cells and G MCF-7 cells. H Measurement of lactate levels in MDA-MB-231 and MCF-7 cells. Let-7a showing inhibition of glycolysis in MDA-MB-231 cells. Effect of let-7a on adipogenesis. I Graphs representing staining of Oil Red O in MCF-7 cells transfected with mimic/antisense of let-7a. Level of significance was tested by Two-way anova in Fig A, B and C, (****p < 0.0001). The data are represented as mean + SD analyzed by unpaired t-test, n = 3. (p < 0.05). (**p < 0.01)