Fig. 2

TMED3 knockdown inhibits progression of osteosarcoma in vitro. A MTT assay was employed to show the effects of TMED3 on cell proliferation of MNNG/HOS and U-2OS cells. B) Colony forming ability of MNNG/HOS and U-2OS cells was detected. C Flow cytometry was performed to detect cell apoptosis of MNNG/HOS and U-2OS cells with or without TMED3 knockdown. D, E The MNNG/HOS and U-2OS cell migration ability was accessed by wound-healing assay (D) and Transwell assay (E). The presented figures are representative data from at least three independent experiments. Data was shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001