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Fig. 4 | Cancer Cell International

Fig. 4

From: TMED3/RPS15A Axis promotes the development and progression of osteosarcoma

Fig. 4

The exploration of downstream underlying TMED3 induced regulation of osteosarcoma. A A Prime View Human Gene Expression Array was performed to identify the differentially expressed genes (DEGs) between shTMED3 and shCtrl groups of MNNG/HOS cells. In the heat map of cluster analysis, each column represents a sample and each row represents a differential gene. The red indicates that the gene expression is upregulated, the green indicates that the gene expression is downregulated, the black indicates that the gene expression is not significantly changed, and the gray indicates that the signal strength of the gene is not detected. B The top 5 DEGs with the largest variation were screened out for further verification by WB. C The lentiviral system mediated knockdown of ITGA6, KIF20A, PTGS2, RPL31, RPS15A and tested the effects on the viability of osteosarcoma cells. D The expression of RPS15A in tumor tissues and normal tissues was evaluated by IHC analysis. E RPS15A expression profiles of tumor (n = 10) and normal (n = 2) samples from osteosarcoma patients was analyzed from GEO database. F The mRNA expression of RPS15A in human osteosarcoma cells (U-2OS, Saos-2, MNNG/HOS, MG-63) and osteoblast cells (hFOB 1.19) was accessed by qPCR. The representative images were selected from at least 3 independent experiments. Data was shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

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