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Fig. 2 | Cancer Cell International

Fig. 2

From: NEDD4L-induced ubiquitination mediating UBE2T degradation inhibits progression of lung adenocarcinoma via PI3K-AKT signaling

Fig. 2

NEDD4L binds to UBE2T and regulates UBE2T levels. A Basal level of NEDD4L and UBE2T protein in lung cell lines. B NEDD4L immunoprecipitated endogenous UBE2T. H1299 cells were transiently transfected with HA-NEDD4L, then cells were lysed and immunoprecipitated with HA antibody, followed by IB with indicated antibodies. C, D Overexpression of NEDD4L decreased the protein level of UBE2T, but had no effect on UBE2T mRNA. H1299 cells were transfected with HA-NEDD4L or vector control, followed by IB (C) or qRT-PCR (D). E, F NEDD4L depletion increased UBE2T protein level, but not mRNA: H358 cells were transfected with siRNA targeting NEDD4L along with control RNAi, followed by IB (E) or qRT-PCR (F)

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Cancer Cell International

ISSN: 1475-2867

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