Fig. 8From: Bioinformatics prediction and experimental verification identify MAD2L1 and CCNB2 as diagnostic biomarkers of rhabdomyosarcomaDownregulation of MAD2L1 and CCNB2 inhibits RMS cell growth, invasion and migration as well as promotes cell cycle arrests and cell death. a EdU assays showed that RMS cell proliferation was detected. Hoechst staining, EdU labeled cell proliferation (red), total cells (blue), siMAD2L1 and siCCNB2 inhibited cell growth (pink). b CCK8 shows the cell proliferation ability. c Apoptosis of RH30 and RD cells transfected with small interfering RNA fragments of MAD2L1 and CCNB2 was detected by TUNEL. DAPI staining, all cells (blue), TUNEL-labeled apoptotic cells (red), siMAD2L1 and siCCNB2 promoted cell apoptosis (pink). d Rate of apoptosis was analysed by flow cytometry. e AO staining apoptotic cells, followed by flow cytometric analysis. f Changes of cell cycle. g, h The migration and invasion capabilities of RMS cells. P-value < 0.05, via independent sample t-testBack to article page