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Fig. 8 | Cancer Cell International

Fig. 8

From: Bioinformatics prediction and experimental verification identify MAD2L1 and CCNB2 as diagnostic biomarkers of rhabdomyosarcoma

Fig. 8

Downregulation of MAD2L1 and CCNB2 inhibits RMS cell growth, invasion and migration as well as promotes cell cycle arrests and cell death. a EdU assays showed that RMS cell proliferation was detected. Hoechst staining, EdU labeled cell proliferation (red), total cells (blue), siMAD2L1 and siCCNB2 inhibited cell growth (pink). b CCK8 shows the cell proliferation ability. c Apoptosis of RH30 and RD cells transfected with small interfering RNA fragments of MAD2L1 and CCNB2 was detected by TUNEL. DAPI staining, all cells (blue), TUNEL-labeled apoptotic cells (red), siMAD2L1 and siCCNB2 promoted cell apoptosis (pink). d Rate of apoptosis was analysed by flow cytometry. e AO staining apoptotic cells, followed by flow cytometric analysis. f Changes of cell cycle. g, h The migration and invasion capabilities of RMS cells. P-value < 0.05, via independent sample t-test

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