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Fig. 2 | Cancer Cell International

Fig. 2

From: The circular RNA circSLC7A11 functions as a mir-330-3p sponge to accelerate hepatocellular carcinoma progression by regulating cyclin-dependent kinase 1 expression

Fig. 2

Description and clinical features of circSLC7A11. a Schematic illustration of circSLC7A11 and the head-to-tail splicing junction site, validated by Sanger sequencing. b Nucleic acid electrophoresis confirmed the presence of circSLC7A11 in HCCLM3 and Hep3B cells. CircSLC7A11 was amplified by divergent primers in cDNA, but not gDNA. GAPDH was used as a negative control. c Relative expression of circSLC7A11 and SLC7A11 mRNA in HCCLM3 and Hep3B cells in the presence or absence of RNase R. d The qRT-PCR product of circSLC7A11 was detected by nucleic acid electrophoresis in the presence or absence of RNase R. GAPDH was used as a negative control. e A nucleocytoplasmic fractionation assay indicated that circSLC7A11 is mainly localized in the cytoplasm of HCCLM3 and Hep3B cells. GAPDH and U6 were used as negative controls. f A fluorescence in situ hybridization (FISH) assay demonstrated the localization of circSLC7A11 in HCCLM3 and Hep3B cells. Blue indicates nuclei stained with DAPI; red indicates circSLC7A11 staining. Scale bar, 100 μm. g Survival curves of HCC patients with low and high expression of circSLC7A11 (n = 93, p = 0.0034). Data are means ± SD (ns = not significant, *p < 0.05; **p < 0.01; ***p < 0.001)

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