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Fig. 6 | Cancer Cell International

Fig. 6

From: The circular RNA circSLC7A11 functions as a mir-330-3p sponge to accelerate hepatocellular carcinoma progression by regulating cyclin-dependent kinase 1 expression

Fig. 6

CircSLC7A11 functioned as an miR-330-3p sponge to regulate CDK1 expression. a Relative mRNA and protein levels of CDK1 detected in HCCLM3 cells transfected with negative control, si-circ, and miR-330-3p inhibitor according to qRT-PCR (upper) and Western blotting (bottom). b Relative mRNA and protein levels of CDK1 detected in Hep3B cells transfected with vector, oe-circ, and miR-330-3p mimic using qRT-PCR (upper) and Western blot (bottom). c A FISH assay showed the cellular localization of circSLC7A11 (red) and miR-330-3p (green) in HCC cells. Scale bar, 100 μm. d An RNA immunoprecipitation assay detected circSLC7A11 and miR-330-3p binding to Ago2. e qRT-PCR showed enrichment of circSLC7A11 and miR-330-3p in a biotin-labeled RNA pulldown assay of HCCLM3 cells. f Schematic illustration of CDK1-3’UTR-WT and CDK1-3’UTR-MUT luciferase reporter vectors. g Relative luciferase activities detected in HEK-293 T cells after co-transfection with CDK1-3’UTR-WT or CDK1-3’UTR-MUT and a mimic or negative control. h Pearson correlation analysis of circSLC7A11, miR-330-3p, and CDK1 expression. n = 93; Data are means ± SD (ns = not significant, *p < 0.05; **p < 0.01; ***p < 0.001)

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