Fig. 4

Knockdown of CCNI2 results in the alteration of downstream proteins. A The DEGs between shCCNI2 and shCtrl groups of SGC-7901cells was identified. In the heat map of cluster analysis, each column represents a sample and each row represents a differential gene. The red indicates that the gene expression is upregulated, the green indicates that the gene expression is downregulated, the black indicates that the gene expression is not significantly changed, and the gray indicates that the signal strength of the gene is not detected. B, C The expression of several selected DEGs of SGC-7901 cells after knockdown of CCNI2 was measured by qPCR (B) and western blot (C). D Pearson correlation analysis indicated a significant positive correlation between CCNI2 and HDGF expression. E Proteins interaction between CCNI2 and CDK1 was determined by Co-IP assay. The presented results were representative of experiments repeated at least three times. Data was represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001