Fig. 5

CRART16 binds to miR-193b-5p as a ceRNA. a Schematic illustration of miR-193b-5p binding sequences in CRART16. b The dual luciferase reporter assay was performed to measure the luciferase activity after co-transfection of 293 T cells with miR-193b-5p mimics or NC and pmiR-RB-Report™-CRART16-WT vector or empty vector. *p < 0.05. c The expression levels of miR-193b-5p in CRART16-overexpressing CRC cells, SW620/5-FU cells and their corresponding control cells were measured by RT-qPCR. U6 was used as an internal reference. Data are presented as the mean ± SD from three independent experiments. **p < 0.01, ***p < 0.001. d, e The viability of SW620-CRART16 and Caco-2-CRART16 cells transfected with miR-193b-5p mimics or NC was detected by CCK-8 assay after treatment with stepwise increasing concentrations of 5-FU for 48 h. The IC50 values of 5-FU were calculated. *p < 0.05, **p < 0.01