Fig. 6

Schematic diagram of the effect of PG on inhibiting autophagy in CCA cells. During autophagy, LC3B and P62 in autophagosomes will be degraded by hydrolase in lysosomes along with the fusion of autophagosomes with lysosomes. STX17, SNAP29 and VAMP8 constitute complex of SNAREs, which plays a key role in this fusion process. When the expression of STX17 and SNAP29 was inhibited by PG, STX17 and SNAP29 could not effectively form SNARE complex with VAMP8. And the fusion of autophagosome with lysosome was then inhibited, which subsequently result in failure of degradation of LC3B and P62