Fig. 1

Inhibition effect of ALO on the proliferation and self-renewal of NSCLC cells. A H1299 and A549 cells were treated with ALO for 24, 48 and 72 h at 0.2, 0.4, 0.8 and 1.6 mM, respectively, cells proliferation was detected by CCK8 assay. *P < 0.05, the ALO group (0.2, 0.4, 0.8 and 1.6 mM) compared to the control group (0 mM, solvent ethanol) at 24 h; #P < 0.05, the ALO group (0.2, 0.4, 0.8 and 1.6 mM) compared to the control group (0 mM, solvent ethanol) at 48 h; &P < 0.05, the ALO group (0.2, 0.4, 0.8 and 1.6 mM) compared to the control group (0 mM, solvent ethanol) at 72 h. B H1299 and A549 cells were treated with the indicated dose of ALO, the colony formation viability was evaluated by clonogenic formation assay. *P < 0.05, compared to the control group (0 mM, solvent ethanol); #P < 0.05, compared to the ALO group (0.4 mM or 0.3 mM). Data are from one experiment representative of three experiments. Two-tailed Student’s test