Fig. 5

Salvianolic acid B inhibits the migration and invasion of hepatocellular carcinoma cells by regulating mortalin. A Western blot assays of E-cadherin, N-cadherin, vimentin, p-STAT3, Ac-STAT3, and GAPDH were performed. MMP2 and MMP9 activities in HCCLM3 cells were investigated by gelatin zymography assays. B HCCLM3 cells were subjected to migration and invasion assays, and migrated and invaded cells were counted with Stat Monitor in Photoshop (mean ± SD, n = 3). ^*P < 0.05, statistically significant difference vs. si-NC cells. ^#P < 0.05, statistically significant difference vs. si-NC cells treated with 100 μM Sal B. C After HCCLM3 cells were treated with NC or 100 μM Sal B for 48 h, the binding of mortalin to RECK was detected by immunoprecipitation. D HCCLM3 cells were transiently transfected with vector or Flag-mortalin for 8 h, and then the cells treated with 0 or 100 μM Sal B were incubated for an additional 24 h in fresh medium with 10% FBS. Western blot assays of E-cadherin, N-cadherin, vimentin, Flag, RECK, p-STAT3, Ac-STAT3, and GAPDH were performed. MMP2 and MMP9 activities were investigated by gelatin zymography assays. E HCCLM3 cells were subjected to the migration assays, and migrated cells were counted with Stat Monitor in Photoshop (mean ± SD, n = 3). ^*P < 0.05, statistically significant difference vs. untreated cells. Bars = 100 μm. ^#P < 0.05, statistically significant difference vs. si-NC cells treated with 100 μM Sal B