Fig. 4

YTHDF2, as a tumor promoter, may lead to a poor prognosis for LUSC patients. A Representative immunoblot showed YTHDF2 overexpression to promote METTL14 upregulation in NCI-H226, not of METTL3. Data are represented by the mean ± SD of three independent experiments. *P < 0.05 vs. the vector group. B and C Using the Pearson correlation statistics, we examine the pairwise gene correlation analysis between YTHDF2 and METTL14, YTHDF2 and METTL3 by TCGA and GTEx expression data of GEPIA. D By a log-rank test for the overall survival (OS) and disease-free survival (DFS) analysis in LUSC, we respectively investigate gene YTHDF2, METTL14, and Snail by the ‘Survival’ tab of GEPIA. E In the immunohistochemical staining results, the overall survival of LUSC patients with pN, pTNM, and YTHDF2 expression was analyzed by A Kaplan–Meier analysis