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Fig. 5 | Cancer Cell International

Fig. 5

From: Hypoxia-mediated YTHDF2 overexpression promotes lung squamous cell carcinoma progression by activation of the mTOR/AKT axis

Fig. 5

Hypoxia specifically induces YTHDF2 overexpression to activate the mTOR/AKT axis in LUSC cells. A RT-qPCR was used to investigate the alterations of mRNA levels of YTHDF2 when LUSC cells were exposed to 1% O2 for 6 h, 16 h, 24 h, 48 h, respectively. B NCI-H226 and SK-MES-1 cells were exposed with 20% O2 or 1% O2 (1% O2, 5%CO2, 94% N2) for 24 h. The protein levels of HIF-1α, YTHDF2, and P-AKT (be phosphorylated at serine 473) were analyzed by western blot. C Using the Pearson correlation statistics, we examine the pairwise gene correlation analysis between HIF-1α and YTHDF2 by TCGA and GTEx expression data of GEPIA. D The cellular growth was analyzed by CCK8 assay at different time points. E The cellular invasion and migration growths were analyzed by transwell assay. F The migration viability was analyzed by wound-healing assay. Data are represented by the mean ± SD of three independent experiments. *P < 0.05 vs. the vector group

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