Fig. 2

GLA induces MM cell apoptosis. A The six MM cell lines (U266, KMS11, OPM2, OCI-MY5, RPMI-8226 and LP1) were treated with GLA (10 μM) for 24 h, and cells were then stained with Annexin V-FITC and PI and analyzed on a BD FACSCalibur™ flow cytometer. B MM cell lines (RPMI-8226 and LP1) were treated with the indicated concentrations of GLA for 24 h, and cells were then stained with Annexin V-FITC and PI and analyzed by flow cytometer. C The levels of apoptosis related gene, Caspase-3 and Bcl-2 in MM cell lines (RPMI-8226 and LP1) treated with 10 μM GLA for 24 h were established by real time qPCR. D The levels of apoptosis related proteins PRAP, pro-caspase-3, and cleaved-caspase-3 and Bcl-2 in MM cell lines (RPMI-8226 and LP1) treated with different concentrations of GLA for 24 h were established by western blot analysis. E RPMI-8226 cells were pre-treated with or without Z-VAD-FMK (10 μM) for 3 h, and then added with the indicated concentrations of GLA (1 μM) for 24 h followed by Annexin V/PI apoptosis assay