Knockdown of C1QTNF6 activates the Acute Phase Response signaling pathway and targets TNF. A Hierarchical clustering analysis heat map of differentially expressed genes. Red indicates upregulation, and green indicates downregulation; black indicates no obvious difference. B The TNF network showed the relationships of predicted upstream regulatory factor, TNF and down-stream molecules in the data set. C Signaling pathways enriched among differentially expressed genes. The Y-axis represents the − log (10) P value for enrichment, with the threshold drawn at P = 0.05.