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Fig. 3 | Cancer Cell International

Fig. 3

From: Cervical cancer-derived exosomal miR-663b promotes angiogenesis by inhibiting vinculin expression in vascular endothelial cells

Fig. 3

Exosomes transport miR-663b to HUVECs and inhibit VCL expression. A qPCR analysis of the relative expression of miR-663b in exosomes harvested from SiHa, HeLa, CaSki and H8 cell lines. B TEM images of exosomes isolated from CaSki cells. C CaSki cell-secreted exosome-positive markers CD63, TSG101, CD81 and GAPDH were detected by Western blot analysis. D Nanoparticle size analysis of CaSki cell-secreted exosomes. E CaSki cells pretreated with PKH67-labeled exosomes for 0, 2, 4 and 8 h were stained with DAPI (blue) for confocal microscopy analysis (magnification ×200). F qRT-PCR assay of miR-663b in HUVECs pretreated with CaSki exosomes, miR-663b-up exosomes, miR-663b-inhibitor exosomes and NC exosomes. G VCL expression in HUVECs treated with CaSki exosomes, miR-663b-up exosomes, miR-663b-inhibitor exosomes and NC exosomes and the corresponding quantitative analysis. *P < 0.05, **P < 0.01 and ***P < 0.001. VCL, vinculin; TEM, transmission electron microscopy; TSG101, tumor susceptibility gene 101

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