Fig. 6

TKTL1 was involved in glycolysis of CC cells by regulating AKT and thus activating PFKFB3 rather than HK2. A RT-qPCR detected the expression of HK-2 in the CC cells after transfection of shRNA-HK-2. B Western blot detected the expression of HK-2 in the CC cells after transfection of shRNA-HK-2. C RT-qPCR detected the expression of PFKFB3 in the CC cells after transfection of shRNA- PFKFB3. D Western blot detected the expression of PFKFB3 in the CC cells after transfection of shRNA-PFKFB3. *p < 0.05, **p < 0.01, ***p < 0.001 vs shRNA-NC. E The effect on the ECAR in SiHa cells after cell transfection. The effect on glucose uptake (F), lactate production (G), and ATP generation (H) in SiHa cells after cell transfection. ***p < 0.001 vs shRNA-TKTL1; #p < 0.05, ##p < 0.01, ###p < 0.001 vs shRNA-TKTL1 + SC-79 + shRNA-NC