Fig. 3

The DEGs in pyrimidine metabolism are overexpressed in GC. A The mRNA expressions of pyrimidine metabolism (NT5E,UPP1 and DPYS) were accessed in six GC cell lines (including HGC-27, MGC-803, SGC-7901, BGC-823, MKN-45 and MKN-28) and one immortalized normal gastric epithelial cell GES-1. B The mRNA expressions of pyrimidine metabolism were also compared between 20 pairs of GC tumor tissues and adjacent non-tumor gastric tissues. C, D The expression of NT5E,UPP1 and DPYS were analyzed by western blot between normal gastric cell and GC cell lines. E–H The expression of NT5E, UPP1 and DPYS was analyzed by western blot and immunochemistry in 20 pairs of GC tumor tissues and adjacent non-tumor gastric tissues. Data are presented as the mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001: ****p < 0.0001