Fig. 5

Re-expression of RAGE with RAGE shRNA restored the level of autophagy and apoptosis induced by ADM in RAGE⁻ Jurkat cells. A (a-1) RAGE was overexpressed in RAGE-knockdown Jurkat cells. RAGE⁻ Jurkat cells were transfected with shRNA-RAGE and shRNA-NC for 2 weeks. Microscopic analysis is shown with 200× magnification. Then cells were subjected to western blotting to verify the knock down of RAGE protein, as shown in a-2. B The transfected cells were treated with ADM (0.4 μM). Western blotting was used to detect the alternation of autophagy. The diagrams of the quantitative data of LC3II/I and P62 in transfected cells were presented. C Cells that stably transfected with shRNA2-RAGE were treated with ADM (0.8 μM),western blotting was used to assess the level of apoptosis. Data are the mean ± standard deviation of three independent experiments. *P < 0.05, **P < 0.01, compared with the RAGE⁻ Jurkat group