Fig. 7
From: Extracellular HMGB1 interacts with RAGE and promotes chemoresistance in acute leukemia cells
The obstruction of HMGB1/RAGE increased sensitivity to chemotherapy in vivo. A 5 × 106 Jurkat cells with stable transfection of control and Rage shRNA as well as mutated acetylation site of HMGB1 were injected into NOD/SCID mouse via intravenous injection to establish a model of leukemia mouse. Low dosage of ADM (1 mg/per mouse) was injected into mouse every other day. The diagram showed us the life span of mouse in different groups. B Bone marrow sediment of mouse was removed and stained with BV421-CD3 before flow cytometry to detect the proportion of leukemia cells in bone marrow. C When mouse come to the endpoint of death, spleen was separated and fixed with 4% paraformaldehyde. Pathological section was made by using hematoxylin–eosin staining. Microscopic analysis is shown with 40× magnification. D Bone marrow sediment of mouse was removed and subjected to transmission electron microscopy to observe autophagosome-like structures (indicated by red triangle)