Fig. 4

YB-1 is the direct target of miR-548ac. a Bioinformatic algorithms predicted miR-548ac might interact 3′UTR of YB-1 at the position of 170–177 and 262–269 nucleotides, the mutant sequences of MUT Vector mentioned below are shown as indicated. b, c After inhibition or overexpression of miR-548ac in the normal or acidic medium, the expression of YB-1 mRNA and protein were measured by qPCR and western blot, respectively. d The pMIR-REPORT luciferase vector containing 3′UTR of YB-1 sequences: the wild type of miR-548ac target sequence (WT) or the corresponding mutant type sequence (MUT) were co-transfected with inhibitors or mimics of miR-548ac in normal or acidic medium. Then the activity of luciferase density was measured