Skip to main content
Fig. 6 | Cancer Cell International

Fig. 6

From: A reciprocal feedback of miR-548ac/YB-1/Snail induces EndMT of HUVECs during acidity microenvironment

Fig. 6

YB-1 activated Snail translation in HUVECs. a In the normal medium, after HUVECs were transfected with miR-548ac mimics alone or along with YB-1 overexpression plasmid, the expression of Snail mRNA and protein was measured by qPCR and western blot, respectively. b In the normal medium, after HUVECs were transfected with miR-548ac inhibitors alone or along with YB-1 siRNAs, the expression of Snail mRNA and protein was measured by qPCR and western blot, respectively. c The HUVECs cells were cultured in the normal, acidic medium or acidic medium transfected with YB-1 siRNAs. Then the Snail mRNA and protein level were measured by qPCR and western blot, respectively. d In an acidic medium, after HUVECs were transfected with miR-548ac mimics alone or along with a YB-1 overexpression plasmid, the expression of Snail mRNA and protein was measured by qPCR and western blot, respectively. e The HUVECs were cultured in the normal and acidic medium, and then both groups were treated with actinomycin D (1 μg/mL) as indicated time, the expression of Snail mRNA was measured by qPCR. f The HUVECs in the normal and acidic medium were both treated with CHX (10 μg/mL), and the level of Snail protein was measured by western blot. g The polysome analysis was performed in both normal and acidic mediums after YB-1 was knockdown in HUVECs. h The HUVECs permeability and transendothelial migration of BxPC-3 cells, as well as the level of Snail protein, were measured when HUVECs were divided into four groups, normal, acidity, acidity with miR-548ac mimics and acidity co-transfected with miR-548ac mimics, and YB-1 overexpression plasmid. i HUVECs permeability and transendothelial migration of BxPC-3 cells as well as the Snail protein level were measured when HUVECs were divided into four groups, normal, acidity, acidity with YB-1 siRNAs, and acidity co-transfected with YB-1 siRNAs and Snail overexpression plasmid

Back to article page