Fig. 4

CircSEC24A directly binds to miR-606 to mediate proliferation and migration of pancreatic cancer cells. A The schematic diagram of circSEC24A which contains MER. B CircRNA-miRNA-mRNA network was performed to predict the potential miRNA and mRNA based on CSCD database. C PCR analysis was performed to confirm the potential binding miRNA. D The schematic diagram showed the wild type binding sequence and the mutant type binding sequence of circSEC24A with miR-606. E Dual luciferase reporter assay was performed to confirm the binding site. F The expression of miR-606 was detected by PCR analysis. G The correlation analysis between circSEC24A and miR-606. H–J Rescue experiments was performed to measure the effect of miR-606 inhibitor combining with si-circSEC24A on proliferation through CCK-8 and EDU assays. Representative images were obtained at ×400 magnification. K–N Rescue experiments was performed to measure the effect of miR-606 inhibitor combining with si-circSEC24A on migration and invasion through transwell and wound healing assays. Representative images were obtained at ×200 magnification