Fig. 5

TGFBR2 is target of miR-606 and promotes pancreatic cancer cell proliferation and migration. A The candidate target of miR-606 was identified by PCR analysis. B The schematic diagram showed the wild type binding sequence and the mutant type binding sequence of TGFBR2 with miR-606. C Dual luciferase reporter assay was performed to confirm the binding site. D PCR analysis was performed to measure the mRNA expression level of TGFBR2 in pancreatic cancer cells transfected with miR-606 mimics or inhibitors. E Western blot was performed to measure the protein expression level of TGFBR2 in pancreatic cancer cells transfected with miR-606 mimics or inhibitors. F–H Rescue experiments was performed to measure the effect of miR-606 mimics combining with TGFBR2 on proliferation through CCK-8 and EDU assays. Representative images were obtained at ×400 magnification. I–L Rescue experiments was performed to measure the effect of miR-606 mimics combining with TGFBR2 on migration and invasion through transwell and wound healing assays. Representative images were obtained at ×200 magnification