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Fig. 5 | Cancer Cell International

Fig. 5

From: Imperatorin induces autophagy and G0/G1 phase arrest via PTEN-PI3K-AKT-mTOR/p21 signaling pathway in human osteosarcoma cells in vitro and in vivo

Fig. 5

IMP triggered autophagy of human osteosarcoma cells. A A visualized autophagy pathway heatmap with relative gene expression level. The expression level of key proteins ULK1 (ATG1), ATG5 and LC3 in autophagy pathway of 143B cells (B) and U2OS cells (C) treated with various concentration of IMP were detected by Western Blot. D LC3B immunofluorescence images of 143B and U2OS cells treated with various concentration of IMP for 24 h. AO staining images of 143B cells treated with blank control (E), 25 μM IMP (F) and 125 μM IMP (G) were photographed by inverted fluorescence microscope. Scale bars in (E–G) equaled 200 μm while that in (D) equaled 50 μm

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