Fig. 7

Inhibition of PTEN rescued the effect of IMP on human osteosarcoma cells. A Inhibition of PTEN reduced IMP-induced inhibitory effect on proliferation of 143B and U2OS cells. AO staining images of 143B cells treated with blank control (B), 125 μM IMP (C) and 125 μM IMP combined with 1 μM Bpv (HOpic) (D) were photographed by inverted fluorescence microscope. The cell cycle distribution map of 143B cells treated with blank control (E), 125 μM IMP (F), 125 μM IMP combined with 1 μM Bpv (HOpic) (G) detected by flow cytometry, and the semi-quantitative statistical chart of them (H). The cell cycle distribution map of U2OS cells treated with blank control (I), 125 μM IMP (J), 125 μM IMP combined with 1 μM Bpv (HOpic) (K) detected by flow cytometry, and the semi-quantitative statistical chart of them (L). The expression level of key proteins AKT, p-AKT, mTOR, p-mTOR, p21, CDK6 and LC3 in 143B cells (M) and U2OS cells (N) exposed to various concentration of IMP and Bpv were determined by Western blot. O A schematic of the effects of IMP on PTEN-PI3K-AKT-mTOR/p21 signaling pathway in human osteosarcoma cells. Scale bars equaled 200 μm. *p  < 0.05; **p  < 0.01; ***p  < 0.001; one-way ANOVA; ⁺  p  < 0.05;  ⁺⁺  p  < 0.01;  ⁺⁺⁺ p  < 0.001, one-way ANOVA; data were mean  ±  SD