Fig. 1

Expression analysis of SCUBE3 and establishment and validation of scube3 knockdown cell model. A SCUBE3 expression in tumour and normal tissues in the TCGA database from UALCAN database. SCUBE3 expression is based on the pa'tients sex, age, body weight, tumour grade and cancer stage. B, C RT-qPCR and western blot analysis of SCUBE3 mRNA and protein expression in normal hepatocytes HL7702 and four HCC cell lines (Bel7404, Bel7402, HepG2, and SMMC7721). D Western blotting was used to detect the expression level of SCUBE3 in the whole protein, cytoplasmic protein, and membrane protein samples of Bel7404 HCC cells. E Bel7404 cells were transfected with control shRNA (shCtrl) and three shSCUBE3 lentiviruses (shSCUBE3-1, shSCUBE3-2, shSCUBE3-3). Green fluorescence protein (GFP) expression illustrated to transfect with lentivirus successfully. F The expression levels of SCUBE3 in the cells were determined through reverse-transcriptase PCR. G–H The protein expression of SCUBE3 was examined by western blotting (n = 3, ns,no significance,*p < 0.05,**p < 0.01, and ***p < 0.001 compared with normal cells)