Fig. 5

CCNE1 silence inhibit Bel7404 cell proliferation, migration, arrested G1/S transition and promote apoptosis. A 5 days of continuous MTT assays. B Knockdown of CCNE1 remarkably enhanced caspase-3/7 activity in the shCCNE1 group. C Knockdown of CCNE1 promoted cell apoptosis. D Analysis of cell cycle profiles after CCNE knockdown in Bel7404 cells. E Colony formation assay using cells infected with CCNE1 lentiviral vector and empty vectors. Representative images were acquired, and the colonies were counted after two weeks. F The inhibition effect of CCNE1 silence on cell migration was assessed by transwell assay. After 24 h of incubation, the cells were fixed and stained. Representative images of transwell chamber assays were acquired, and quantification of the number of cells which migrated through the basement membrane (n = 5 per group) (*p < 0.01, **p < 0.01,***p < 0.001 compared with shCtrl)