Fig. 6
![Fig. 6](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12935-021-02402-z/MediaObjects/12935_2021_2402_Fig6_HTML.png)
Overexpression of CCNE1 in SCUBE3 knockdown Bel7404 cells regulates proliferation and apoptosis. A MTT proliferation assay. B caspase 3/7 activity assay. C Detection of apoptosis in cells with both SCUBE3 knockdown and CCNE1 overexpression and control cells by the Annexin V-APC/PI double staining assay and, D cell cycle analysis of cells with both SCUBE3 knockdown and CCNE1 overexpression and control cells. E SCUBE3 knockdown cells were treated with overexpression lentivirus of CCNE1 for 72 h, Cell lysates were analyzed by immunoblotting using the indicated antibodies (*p < 0.01, **p < 0.01,***p < 0.001)