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Fig. 6 | Cancer Cell International

Fig. 6

From: SCUBE3 downregulation modulates hepatocellular carcinoma by inhibiting CCNE1 via TGFβ/PI3K/AKT/GSK3β pathway

Fig. 6

Overexpression of CCNE1 in SCUBE3 knockdown Bel7404 cells regulates proliferation and apoptosis. A MTT proliferation assay. B caspase 3/7 activity assay. C Detection of apoptosis in cells with both SCUBE3 knockdown and CCNE1 overexpression and control cells by the Annexin V-APC/PI double staining assay and, D cell cycle analysis of cells with both SCUBE3 knockdown and CCNE1 overexpression and control cells. E SCUBE3 knockdown cells were treated with overexpression lentivirus of CCNE1 for 72 h, Cell lysates were analyzed by immunoblotting using the indicated antibodies (*p < 0.01, **p < 0.01,***p < 0.001)

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