Fig. 7

SCUBE3 binds to the TGFβIIR. A Co-localization of SCUBE3 and TGFβIIR. HepG2 and Bel7404 cells were stained with antibodies against SCUBE3 and TGFβIIR, followed by incubation with FITC-conjugated donkey anti-rat or anti-mouse IgG. The cells were visualized using a confocal microscope. The yellow areas represent protein co-localization. B The TGFβ pathway is significantly downregulated after SCUBE3 knockdown. C Co-immunoprecipitation assay results indicate that SCUBE3 interacts with TGFβIIR. D Western blot analysis of AKT and p-AKT expression in SCUBE3 knockdown cells and control cells. E Western blot analysis of GSK3β and p-GSK3β expression in SCUBE3 knockdown cells and control cells