Fig. 3
From: miR-378d suppresses malignant phenotype of ESCC cells through AKT signaling
miR-378d participates in EMT, migration, and invasion of ESCC cells. a Phalloidin-FITC labeling the F-actin of TE-1-miR-NC (20 nM), TE-1-miR-378d-in (20 nM), KYSE150-miR-NC (20 nM), and KYSE150-miR-378d-in (20 nM) cells. b–d, Transwell assay was performed to detect the cell migration and invasion ability of ESCC cells with high or low expression of miR-378d. Random selection of five fields, ***P < 0.001. e 3D tumor sphere formation, suspension. f 3D tumor sphere formation, in Matrigel. g HE staining for KYSE-150 cells cultured on 3D Alvetex®, a highly loose crosslinked polystyrene scaffold, to detect the depth of cell invasion