Fig. 2

The PAF-AH 1B2 mediated the abnormal proliferation and migration in ovarian cancer cell. Compared the expression of PAF-AH IB2 subunits in ovarian cancer cell lines lysate by Western blot (A). Validated the PAF-AH 1B2 stable knockdown effect in the ovarian cancer cells by Western blot (B). The comparisons were performed between PAF-AH IB2 and control in the clonogenicity in soft agar (C), cell proliferation in 7 days (D), wound healing migration (E). For the dynamic proliferation test, cells (MCAS, SKOV3, 2.0 × 10⁴ cells/well) were planted by duplicate in the indicated ECM-coated E plates; a non-coated well was used as a negative control (F). Cell proliferation curves were monitored through the xCELLigence system (left panel). The rates of cell proliferation over 24 h (slope) were monitored using the RTCA software (right panel). For dynamic migration assay, cells (MCAS, SKOV3, 2.0 × 10⁴ cells/well) were planted by duplicate in the upper chambers of CIM plates, and the lower chambers were contained with 10% FBS (G). The migration curves were monitored through the xCELLigence system (left panel) and the migration rates over 24 h (Slope) were processed using the RTCA software (right panel). A representative experimental result was generated from three independent experiments. **P < 0.01 and ***P < 0.001 as compared to control cells expressing a scramble shRNA control, paired t test