Figure. 5
From: Platelet-activating factor acetyl hydrolase IB2 dysregulated cell proliferation in ovarian cancer
Overexpression of PAF-AH IB2 caused the Caspase-8 activation and normal ovarian epithelium died. Human PAF-AH 1B2 WT transiently over-expression caused the HOSE sick and quickly died after 48-h transfection (A). The blank vector linked with GFP was used as control. The protein sequence comparison between human and mouse (B). Detection Caspase-8 activation with transfected with mouse PAF-AH 1B2 WT or enzymatic active site mutant (E39D, S48C) in living HOSE (C). Western blot validated the Caspase-8 activation and cleavage in the HOSE with mouse PAF-AH 1B2 WT or mutant transfection (D). *P < 0.05 and ***P < 0.001 represented as compared with control