Fig. 1

Cordycepin inhibits osteosarcoma cell viability. a The chemical structure of cordycepin; b CCK-8 assay showing cordycepin inhibits osteosarcoma cell (U2OS, U2R, SAOS2, MNNGHOS, 143B, MG63, and SJSA1) viability in a time- and dose-dependent manner. Osteosarcoma cells were treated with various concentrations of cordycepin for 24, 48, and 72 h; c Cordycepin reduces colony formation of osteosarcoma cells. Colony-formation abilities of U2OS and 143B osteosarcoma cells were examined after cordycepin treatment for 14 days. Representative images and quantification of the colony formation results are shown. The data are expressed as the mean ± SD of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001, by Student’s t-test