Fig. 2

Cordycepin induces osteosarcoma cell apoptosis. a Annexin V/PI staining of U2OS, 143B osteosarcoma cells, and normal osteoblast cell hFOB treated with or without cordycepin at 100, 200, and 400 µM for 48 h was detected and analyzed by flow cytometry. Representative images and quantification of the flow cytometry analysis results are shown; b Western blotting analysis of the expression levels of cleaved PARP, Bax, and Bcl-2 in both U2OS and 143B cells following a 48-h treatment with cordycepin at each designated concentration. The data are expressed as the mean ± SD of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001, by Student’s t-test