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Fig. 3 | Cancer Cell International

Fig. 3

From: Cordycepin augments the chemosensitivity of osteosarcoma to cisplatin by activating AMPK and suppressing the AKT signaling pathway

Fig. 3

Cordycepin enhances the sensitivity of osteosarcoma cells to cisplatin to inhibit osteosarcoma cell proliferation and induce osteosarcoma cell apoptosis. a CCK-8 assay showing the viability of osteosarcoma cell (U2OS, U2R, SAOS2, MNNGHOS, 143B, MG63, and SJSA1) treated with cordycepin and cisplatin at each designated concentration for 48 h. The fraction affected (Fa)-combination index (CI) plots showing that cordycepin exhibited an apparently synergistic effect when combined with cisplatin (CI < 1); b Colony formation experiments showing the colony numbers of U2OS and 143B cells following treatment with cordycepin (100 µM), cisplatin (0.5 µM), or their combination (100 µM cordycepin and 0.5 µM cisplatin). Representative images and quantification of the colony formation results are shown; c Annexin V/PI staining of osteosarcoma cells (U2OS, 143B) treated with cordycepin (200 µM), cisplatin (1 µM), or their combination (200 µM cordycepin and 1 µM cisplatin) for 48 h was detected and analyzed by flow cytometry. Representative images and quantification of the flow cytometry analysis results are shown; d, e Western blotting analysis of the expression levels of cleaved PARP, Bax, and Bcl-2 in both U2OS and 143B cells following a treatment with cordycepin (200 µM), cisplatin (1 µM), or their combination for 48 h. The combination therapy of both cordycepin and cisplatin enhanced the apoptosis of osteosarcoma cells, compared to treatment with each drug alone. The data are expressed as the mean±SD of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001, by Student’s t-test

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