Fig. 5

Cordycepin and cisplatin synergistically inhibit osteosarcoma cell invasion by downregulating MMP-2 and MMP-9 expression. a Transwell assay showed that the invasion abilities of U2OS and 143B cells were inhibited following treatment with cordycepin (100 µM), cisplatin (0.5 µM), or their combination for 48 h. Representative images and quantification of the transwell results are shown; b Western blotting analysis showing the protein expression levels of MMP-2 and MMP-9 in U2OS and 143B cells treated with vehicle (Control), cordycepin (200 µM), cisplatin (1 µM), or their combination for 48 h. The data are expressed as the mean ± SD of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001, by Student’s t-test, scale bars = 100 μm