Fig. 5

YES1 regulates autophagy in cisplatin-resistant ovarian cancer cells. A KEGG pathway enrichment analysis of the top 500 genes with high YES1 expression in the ovarian cancer cohort from TCGA database. B LC3B expression was increased in both A2780-DDP and SKOV3-DDP cells compared with A2780 and SKOV3 cells. C Inhibiting miR-133a significantly enhanced LC3B expression in SKOV3 and A2780 cells. D Transfection with the miR-133a mimic reduced LC3B expression in A2780-DDP and SKOV3-DDP cells. E YES1 knockdown reduces LC3B expression in SKOV3-DDP and A2780-DDP cells. F YES1-OE increases LC3B expression in SKOV3 and A2780 cells. G Autophagic flux was enhanced after transfection with mRFP-GFP-LC3 adenovirus in YES1-OE A2780 cells. H Autophagic flux was decreased after transfection with mRFP-GFP-LC3 adenovirus in siYES1 A2780-DDP cells. I Autophagic antagonist-chloroquine reverses cisplatin resistance caused by YES1-OE in A2780 cells. J The autophagy agonist rapamycin weakens cisplatin sensitivity caused by siYES1 in A2780-DDP cells. For comparisons, Student’s t test was performed; *p < 0.05, **p < 0.01