Fig.4

miR-216a-5p suppresses FDFT1 expression by binding to its mRNA 3’UTR. a Schematic illustration of miRNAs predicted by five online databases, including miRanda, miRDB, miRMap, PITA and Targetscan. b WB analysis to show FDFT1 expression in MGC-803 and HGC-27 cells transfected with NC mimics, miR-216a-5p mimics, miR-370-3p mimics, miR-548c-3p mimics and miR-607 mimics. c Schematic illustration of the wild-type (red) or mutated (green) sequences of the binding sites of miR-216a-5p and FDFT1 mRNA 3’UTR. d, e Luciferase reporter assay to investigate the relative activities in cell with co-transfected with empty plasmids, plasmids with the wildtype or mutated sequences of FDFT1 mRNA 3’UTR and NC mimics and miR-216a-5p mimics in MGC-803 (d) and HGC-27 cells (e), respectively. f WB analysis to show FDFT1 expression in MGC-803 and HGC-27 cells co-transfected with NC mimics or miR-216a-5p mimics and overexpression plasmids of FDFT1. Data are presented as means ± SD. **P < 0.01