Fig. 3

STK25 promotes HCC cell growth. a The cell line expression matrix of liver tumors was obtained from the CCLE dataset. The horizontal axis in the figure represents the expression of STK25, the ordinate is for different cell lines, the size of the dot in the figure represents the level of expression, and the different colors also represent the level of expression. b Expression of STK25 mRNA in human normal liver HL-7702 (L02) and 4 HCC cell lines (HepG2, Huh7, HLF, SMMC-7721) was measured by qRT-PCR. c–e Cell viability assay and flow cytometry. depletion of STK25 significantly inhibits cell proliferation and promotes apoptosis. f, g Wound healing assay. Detection of two time points (0 h, 24 h). The area within the white line is the measured area. Knockdown of STK25 inhibits migration in cell lines. h Transwell Invasion assay. Representative images of invasive cells from control and STK25-silenced group. i Quantification of cells indicates the invasion ability of STK25-silenced is decreased. CON, control. Data are expressed as mean ± SD. ^*, p < 0.05. ^**, p < 0.01. ^***, p < 0.001. Scale bars, 40 μm