Fig. 4
From: STK25 enhances hepatocellular carcinoma progression through the STRN/AMPK/ACC1 pathway
STK25 expression regulates lipid metabolism pathway in HCC cell lines. a Significant KEGG pathway terms of DEGs, including fatty acid degradation pathway and fatty acid metabolism pathway. b, c Kaplan–Meier analysis of overall survival according to the expressions of fatty acid metabolism genes in TCGA data using GEPIA online tool without false discovery rate (FDR) adjustment. The boxes with framed indicated significant results (p < 0.05). d Pearson correlation between STK25 and AMPK, ACC1, ACLY. e The levels of genes related to lipid metabolism, including p-AMPK, AMPK, ACC1, ACLY were determined in controls, STK25-silenced and STK25-overexpressed HCC cells by western blot. f Quantification of western blot for genes related to lipid metabolism. CON, control. sh, knockdown. flag, overexpression. Data are expressed as mean ± SD. *, p < 0.05. **, p < 0.01. ***, p < 0.001. Scale bars, 40 μm