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Fig. 4 | Cancer Cell International

Fig. 4

From: PHD finger protein 20-like protein 1 (PHF20L1) in ovarian cancer: from its overexpression in tissue to its upregulation by the ascites microenvironment

Fig. 4

PHF20L1 isoform expression in SKOV-3 cells under stimulation with EOC ascites. Western blot assays were performed in three independent experiments to analyze PHF20L1 expression, and a representative image is shown in a. Densitometric analyses, PFS and OS were performed and are shown in b, c and d graphs that correspond to "a", "b", and "c" isoforms, respectively, after 24 or 48 h of stimulation with ascites. For the PFS and OS curves, only 48-h expression values were reported. Statistical analysis was performed using the GraphPad program. Significant differences are shown and correspond to ***ρ < 0.0001. GAPDH was used as a loading control

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