Fig. 2
From: Annexin A1 promotes the progression of bladder cancer via regulating EGFR signaling pathway
ANXA1 knockdown impairs proliferation, colony formation, and the G1-to-S phase transition in BLCA cell lines. A ANXA1 expression levels in high ANXA1 samples compared with their low ANXA1 counterparts among 414 BLCA samples in the TCGA cohort. The optimal cutoff value of low and high ANXA1 was calculated by X-tile software. B GSEA analysis of proliferation and cell cycle target gene sets based on the TCGA cohort. C Western blot of ANXA1 in BLCA cell lines transfected with negative control sh-RNA and three different sh-RNAs specific to ANXA1. Ctrl: normal cells; NC: cells transfected negative control sh-RNA; sh-ANXA1#1–3: cells transfected three different sh-RNAs specific to ANXA1. D, E A CCK-8 assay was conducted to detect the effect of ANXA1 on cell viability. F, G DNA synthesis in BLCA cells of the NC and sh-ANXA1 groups was examined by EdU assay. H Colony formation assay of NC and sh-ANXA1 groups. I Cell cycle assay of NC and sh-ANXA1 groups. *p < 0.05, ** p < 0.01, *** p < 0.001