Fig. 8

Knockdown of ANXA1 inhibits tumor growth in subcutaneous xenograft models. A Gross appearance of tumors from NCG mice injected with 5637 NC or 5637 sh-ANXA1 cells. B Tumor growth was monitored for 30 days. The tumor volume of each group is shown as a line chart. C Individual value plot shows the tumor weights of the NC and sh-ANXA1 groups. D Representative IHC staining patterns of ANXA1, P-EGFR and Ki-67 in each group are shown (original magnification 200 ×) (scale bar: 50 μm). E Quantification of the number of Ki-67 positive cells in each group. F Proposed possible mechanism of ANXA1 functions in BLCA. ANXA1 promotes EGFR expression and inhibits P-EGFR degradation to activate EGFR signaling and its downstream pathways, thereby facilitating the proliferation, invasion and migration of BLCA cells