Fig. 7

Expression of epigenetic chromatin modification enzymes after RAN treatment. Stomach cells of mice treated with RAN + lime for 0, 100 and 300 days and analyzed by qRT-PCR for A histone-lysine-N-methyltransferase 2A (KMT2A), B histone acetyltransferase (KAT2A), C demethylase (KDM4C), D histone deacetylation (HDAC3), E EP-300/KAT3B and F PCAF/KAT2B gene expression. Data are the mean ± SEM of a representative experiment performed in untreated control (N = 4) and treated (N = 4 in each category). The values are normalized to the respective GAPDH values. Data were analysed using one-way ANOVA with Tukey’s multiple comparison post-tests. P values are shown in all and considered significant when the values are less than 0.05. G Representative immunohistochemical staining (40x) of mouse stomach shows that Histone-lysine acetyltransferase (KAT2A) positive cells increase with the treatment duration. Magnification (100x) of the marked area is shown. H Scatterplot of H-scores based on IHC for KAT2A-positive cells. The magnification of all these images is 40x. Magnification (100x) of the marked area is shown