Fig. 4

WNT and PTEN/Akt signalling pathways were markedly inhibited by the OTX015/WT-161 combination therapy, resulting in synergistic anticancer effects. A Under various drug conditions, the main proteins of five classical pathways, WNT, mTOR, NF-kB, JAK-STAT and MAPK, were examined by western blotting. B The effects of OTX015 and/or WT-161 on multiple genes in the WNT pathway were analysed by qPCR. C The western blotting results of PTEN, p-GSK3β and GSK3β. Actin was used as a loading control. Data are means ± SD (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001, compared to DMSO. ns not significant