Fig. 5

The oncogenic effect of SHC4 is relied on STAT3 activation. A, B HepG2 and Huh7 stably overexpressing SHC4 cells were transiently transfected siRNA targeting STAT3 or treated with Stattic (2 µmol/L). Cell viability of indicated cells were measured by CCK-8 assay. Knockdown of STAT3 or Stattic treatment reversed the increased proliferation induced by SHC4 overexpression in HepG2 and Huh7 cells. ***P < 0.001, Student t test. C, D Indicated cells were subjected to transwell assay to assess cell invasive and migrative abilities. Knockdown of STAT3 or Stattic exposure significantly attenuated the enhancement in cell migration and invasion induced by SHC4 overexpression. ***P < 0.001, Student t test. E Western blot was performed using specific antibodies for the p-STAT3, STAT3, c-Myc, cyclin D1, P21, E-cadherin, occluding and Slug in HepG2 and Huh7 stably overexpressing SHC4 cells with or without Stattic treatment. F HepG2 and Huh7 stably overexpressing SHC4 cells were transiently transfected siSTAT3 or siCON, then the expression of indicated markers were analyzed by western blotting