Fig. 6

SHC4 overexpression enhances tumor growth in vivo, which could be blocked by Stattic. A Huh7 cells stably transfected with control or SHC4 were injected into the flank of nude mice. Photos for xenograft tumors isolated from nude mice 21 days post-inoculation. B Tumor volume and weight were compared between the two groups at the end of experiment. **P < 0.01, Mann–Whitney U test. C Huh7 cells stably transfected with control or SHC4 were injected into the flank of nude mice. After the tumors grew to 3–5 mm in diameter, mice were treated with Stattic (50 mg/kg, three times per week). Photos for xenograft tumors isolated from nude mice 30 days post-treatment. D Dot plots show the volume and weight of indicated tumors. ns no significance, Mann–Whitney U test. E The expression of SHC4, Ki67 and E-cadherin in indicated subcutaneous xenografts was determined by IHC. Scale bar, 50 μm. F The levels of SHC4, p-STAT3, STAT3, c-Myc, cyclin D1, p21, E-cadherin, Occludin and Slug were analyzed by western blotting from three representative xenograft tumors in each group